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KMID : 0545120210310081079
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Journal of Microbiology and Biotechnology
2021 Volume.31 No. 8 p.1079 ~ p.1087
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Modulation of Inflammatory Pathways and Adipogenesis by the Action of Gentisic Acid in RAW 264.7 and 3T3-L1 Cell Lines
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Kang Min-Jae
Choi Woo-Suk
Yoo Seung-Hyun
Nam Soo-Wan
Shin Pyung-Gyun
Kim Keun-Ki
Kim Gun-Do
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Abstract
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Gentisic acid (GA), a benzoic acid derivative present in various food ingredients, has been shown to have diverse pharmaceutical activities such as anti-carcinogenic, antioxidant, and hepatoprotective effects. In this study, we used a co-culture system to investigate the mechanisms of the anti-inflammatory and anti-adipogenic effects of GA on macrophages and adipocytes, respectively, as well as its effect on obesity-related chronic inflammation. We found that GA effectively suppressed lipopolysaccharide-stimulated inflammatory responses by controlling the production of nitric oxide and pro-inflammatory cytokines and modulating inflammation-related protein pathways. GA treatment also inhibited lipid accumulation in adipocytes by modulating the expression of major adipogenic transcription factors and their upstream protein pathways. Furthermore, in the macrophage?adipocyte co-culture system, GA decreased the production of obesity-related cytokines. These results indicate that GA possesses effective anti-inflammatory and anti-adipogenic activities and may be used in developing treatments for the management of obesity-related chronic inflammatory diseases.
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KEYWORD
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Gentisic acid, anti-inflammatory effect, anti-adipogenic effect, co-culture system
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